The Firefly Lab chapter is the final chapter of The Last of Us. Joel wakes up in the hospital and learns the Fireflies plan to kill Ellie to extract a vaccine. Joel must fight through the hospital, reach the operating room, rescue Ellie, and escape.
This walkthrough covers all combat encounters, collectibles, and the final decision.
What Happens When Joel Wakes Up?
Joel regains consciousness on a couch. Marlene sits nearby and explains the situation.
Marlene’s revelation:
- To create a vaccine, the Fireflies must remove Ellie’s brain along with the mutated fungus
- Ellie’s life must be sacrificed to save humanity
- Marlene leaves after delivering this information
Escape sequence:
- Break free from the guard holding Joel
- Interrogate the guard
- Learn the operating room location from the interrogation

How to Fight Through the Lower Corridors?
After escaping, Joel must navigate hospital corridors filled with Firefly guards.
| Area | Enemy Count | Key Items |
|---|---|---|
| First corridor | 2 guards | Eliminate both |
| Around corner | 4 guards | Assault Rifle drops from enemies |
| Main hall | Large group of guards | Fight to West Wing door |
West Wing approach:
- Enter the hall and locate the highlighted West Wing door
- Hide behind the table on the right
- Wait for 3 guards to run past
- Kill remaining enemy
- Proceed to the stairwell
- Lock the doors behind Joel
Where to Find Audio Recording #80?
Climb to the upper floor after locking the stairwell doors.
Audio Recording #80 is on the table on the right side of the upper floor.
Where to Find Marlene’s Recording #81?
Enter the room on the right side of the upper floor.
Marlene’s Recording #81 is inside this room.
Where to Find Shiv Door #13 and Firefly Pendant #30?
Shiv Door #13 is located at the large table on the upper floor. This is the final shiv door in the game.
Shiv Door #13 contents:
- 10 pills
- Firefly Pendant #30 (30/30 total pendants – final pendant)
Use a shiv to unlock the door at the large table.
Where to Find Marlene’s Diary #82?
After unlocking Shiv Door #13, enter the tent on the upper floor.
Marlene’s Diary #82 is inside the tent and can be read.
How to Fight the Armored Squad?
Beyond the tent, a squad of riflemen wearing body armor guards the path forward.
Combat strategy:
- Eliminate several guards using stealth before engaging
- Armored enemies require more damage to kill
Progression:
- Eliminate guards (stealth recommended for initial kills)
- Run through to the next set of doors
- Barricade the doors after passing through
Where to Find Marlene’s Recording #83?
Kick open the doors on the right after barricading the previous doors.
Marlene’s Recording #83 is on the table in this room.
How to Reach Ellie in the Operating Room?
Enter the operating room after collecting Recording #83.
Operating room encounter:
- The head surgeon refuses to hand over Ellie
- Kill the head surgeon to proceed

The alarm activates immediately after killing the surgeon.
How to Escape the Hospital with Ellie?
After the alarm sounds, Joel must carry Ellie out of the hospital.
Escape route:
- Carry Ellie through the corridors
- Descend using the elevator to reach the basement
How Does the Final Encounter with Marlene End?
Marlene appears in the basement for the final confrontation.

Final choice:
- Marlene attempts one last time to convince Joel to leave Ellie
- Joel kills Marlene
- Chapter and game end after this decision
| All Collectibles in Firefly Lab | Location | Type |
|---|---|---|
| Audio Recording #80 | Right table (upper floor) | Artifact |
| Marlene’s Recording #81 | Right room (upper floor) | Artifact |
| Shiv Door #13 | Large table (upper floor) | Final shiv door |
| 10 pills | Inside Shiv Door #13 | Consumable |
| Firefly Pendant #30 | Inside Shiv Door #13 | Final pendant (30/30) |
| Marlene’s Diary #82 | Tent (upper floor) | Artifact |
| Marlene’s Recording #83 | Table after barricaded doors | Artifact |
| Key Combat Encounters | Location | Enemy Type and Count |
|---|---|---|
| First corridor | After interrogation | 2 guards |
| Corner area | Past first corridor | 4 guards |
| Main hall | Before West Wing | Large guard group |
| Stairwell approach | West Wing entrance | 3 running guards + 1 stationary |
| Armored squad | Beyond tent | Multiple riflemen with body armor |
| Operating room | Final room before escape | Head surgeon |
| Basement | Elevator exit | Marlene (final encounter) |
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